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KMID : 0861020150300040101
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Korea Journal of Herbology
2015 Volume.30 No. 4 p.101 ~ p.108
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Antioxidant and Hepatoprotective Effects of Hydroponic-cultured Ginseng Folium by fermentation
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Lee Ah-Reum
Park Jae-Ho
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Abstract
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Objectives: Positive effects of Ginseng has great research attentions such as anticancer, anti-diabetic, antiaging, liver, immune function, CNS, etc. In this study, we investigated Hydroponic-cultured Ginseng Folium fermented by Bacillus subtilis to establish fermentation conditions for enhancing functionality.
Methods: Ginseng Folium were cultivated hydroponic-cultured and were extracted with methanol. We inoculate Bacillus subtilis for fermentation by adding to 0%, 3% and 5% sugar respectively and checked antioxidant activities, total phenolic content and total saponin content in 2 days intervals during 11 days. The antioxidant activities were studied by the 1,1-diphenyl-2-picryl hydrazyl(DPPH) radical, 2, 2``-Azino-bis(3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt(ABTS) radical scavenging assay and Reducing power assay. We analyzed the Total phenol content, crude saponin content and ginsenoside content. Moreever, Hepatoprotective effects by Glutamic oxaloacetic transaminase(GOT) and Glutamic pyruvic transaminase(GPT) in Sprague-Dawley rat.
Results: The results of DPPH and ABTS were 66.89% and 96.72%, respectively. The reducing power was resulted in optical density of 0.7312 with 3% sugar after 9 days of fermentation. and the concentration at 200 ㎍/㎖. Total phenol content was 36.92㎎/g with 3% sugar after 9 days of fermentation, in which crude saponin content wasn``t changed, and ginsenoside content such as Rg3, Re and Rb was increased. Activities of GOT and GPT concentration were decreased in rat.
Conclusions: This study suggests that hydroponic-cultured Ginseng Folium fermented by Bacillus subtilis in 9 days showed significant efficacy of hepato-protection as well as antioxidant compared to the others. In addition, it shows not only improved value but also utilized hydroponic-cultured Ginseng Folium by fermentation.
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KEYWORD
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Ginseng, Fermentation, Antioxidant, Ginsenoside, Hepatoprotective effect
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